Calculate amplification efficiency and fold changes per cycle from the slope of your qPCR standard curve.
Standard Curve Data
Amplification Efficacy
Enter slope to calculate efficiency
About this calculator
Overview
qPCR efficiency describes how well the target DNA is doubled in each cycle. A perfect efficiency of 100% means the template doubles exactly once per cycle.
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Pro Tips
Standard curve slope of -3.32 corresponds to 100% efficiency.
Acceptable assays typically fall between 90% and 110% efficiency.
Look for an R² value > 0.99 for a reliable standard curve.
Efficiencies > 110% often indicate PCR inhibitors or primer-dimer formation.
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Fun Facts
"Efficiency > 100% often indicates primer-dimers or inhibitors (paradoxically)."
"The 'MIQE' guidelines recommend reporting efficiency for all qPCR publications."
"A perfect doubling (100% eff) implies exactly 2 copies produced per template per cycle."
