Calculate enzyme activity and specific activity based on substrate conversion.
Reaction Data
Activity Metrics
Enter substrate conversion and time to calculate activity
About this calculator
Overview
Enzyme activity is a measure of the quantity of active enzyme present and is thus dependent on conditions, which should be specified. One unit (U) is defined as the amount of enzyme that catalyzes the conversion of 1 micro mole of substrate per minute.
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Pro Tips
1 U = 1 µmol/min.
Specific Activity = Enzyme Activity / Total Protein Mass.
Ensure time is in minutes for standard Units (U).
Activity measurements should specify assay conditions (temperature, pH, substrate).
Enzyme activity can be inhibited by competitive or non-competitive inhibitors.
Store enzymes at low temperatures to maintain activity.
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Fun Facts
"The katal (kat) is the SI unit for enzyme activity, but U is more commonly used in biochemistry."
"Specific activity is a measure of enzyme purity; it increases during purification."
"Enzyme activity is strongly dependent on temperature and pH."
"Enzymes can accelerate reactions by factors of up to 10^20 compared to uncatalyzed reactions."
"The Michaelis-Menten model describes how enzyme activity depends on substrate concentration."
"Most enzymes have optimal activity at body temperature (37°C) and physiological pH (around 7.4)."
"Enzymes are proteins that act as biological catalysts, remaining unchanged after the reaction."
Formula: Activity = Substrate / Time
Factors Affecting Enzyme Activity
Typical enzyme activity vs temperature (bell-shaped curve)
