Resuspension Calculator

Determine the exact buffer volume needed to achieve a target concentration from a dry solute.

Solute & Target

Solute Amount (Mass/Moles)

Unit

Target Concentration

Unit

Ensure units are compatible (e.g., nmol with µM, or µg with µg/µl).

Calculation Results

Enter parameters to see results

About this calculator

Overview

Resuspension is the process of dissolving a dry pellet of DNA, RNA, or protein in a specific volume of buffer to reach a desired concentration.

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Pro Tips

Fun Facts

"1 µM is equivalent to 1 pmol/µL."
"Resuspending 50 nmol in 500 µL results in a 100 µM solution."
"Nuclease-free water or TE buffer are common resuspension liquids."
"Centrifuge stock tubes (like oligonucleotides) before opening to ensure pellet is at the bottom."
"Most primers are shipped dry to improve stability during transport."
"Accurate pipetting is crucial when working with such small volumes."

Formula: Volume = Amount ÷ Concentration