Resuspension Calculator

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Calculate the volume of buffer required to resuspend a known mass or amount of sample to reach a target concentration.

Sample Details

Sample Mass

Target Concentration

Buffer Requirements

Buffer Volume to Add10000.00uL

Buffer Volume vs. Target

How required volume changes with target concentration.

About this calculator

Overview

This calculator helps in preparing solutions by determining how much liquid (buffer or solvent) to add to a specific amount of dry solute (like DNA, RNA, or protein) to achieve a desired concentration. Resuspension is a critical step in molecular biology workflows, particularly for nucleic acids that arrive lyophilized (freeze-dried) from synthesis.

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Pro Tips

For primres, Volume (µL) = nmol * 10 to get 100 µM concentration.
For nucleic acids, prefer TE buffer over water to maintain integrity.
Gently pipette up and down or use low-speed vortexing - vigorous mixing can shear DNA.
After resuspension, briefly centrifuge the tube to collect any droplets from the lid.

Fun Facts

"The choice of buffer can dramatically affect DNA stability - TE buffer (10 mM Tris-HCl pH 8.0 / 1 mM EDTA) protects DNA from degradation better than water alone."
"EDTA in TE buffer chelates metal ions that would activate DNases, while Tris maintains optimal pH to prevent acid hydrolysis of DNA."
"This calculation assumes the solute volume is negligible compared to the buffer volume, which is valid for small masses."
"A rule of thumb for primers: adding 10x the nmol amount in microliters gives a 100 µM stock solution."

Formula: V_{buffer} = \frac{Mass}{C_{target}}